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Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates
Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in transduced target cells, from which a transduction...
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| Publicado no: | Mol Ther Methods Clin Dev |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4756768/ https://ncbi.nlm.nih.gov/pubmed/26942209 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtm.2016.5 |
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