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Hydride Transfer in DHFR by Transition Path Sampling, Kinetic Isotope Effects, and Heavy Enzyme Studies

Escherichia coli dihydrofolate reductase (ecDHFR) is used to study fundamental principles of enzyme catalysis. It remains controversial whether fast protein motions are coupled to the hydride transfer catalyzed by ecDHFR. Previous studies with heavy ecDHFR proteins labeled with (13)C, (15)N, and non...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Biochemistry
Egile Nagusiak: Wang, Zhen, Antoniou, Dimitri, Schwartz, Steven D., Schramm, Vern L.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2015
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4752833/
https://ncbi.nlm.nih.gov/pubmed/26652185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b01241
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