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Hydride Transfer in DHFR by Transition Path Sampling, Kinetic Isotope Effects, and Heavy Enzyme Studies

Escherichia coli dihydrofolate reductase (ecDHFR) is used to study fundamental principles of enzyme catalysis. It remains controversial whether fast protein motions are coupled to the hydride transfer catalyzed by ecDHFR. Previous studies with heavy ecDHFR proteins labeled with (13)C, (15)N, and non...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Wang, Zhen, Antoniou, Dimitri, Schwartz, Steven D., Schramm, Vern L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4752833/
https://ncbi.nlm.nih.gov/pubmed/26652185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b01241
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