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Accurate measurement of (3)J(HNHα) couplings in small or disordered proteins from WATERGATE-optimized TROSY spectra

Provided that care is taken in adjusting the WATERGATE element of a (1)H-(15)N TROSY-HSQC experiment, such that neither the water magnetization nor the (1)H(α) protons are inverted by its final 180° pulse, (3)J(HNHα) couplings can be measured directly from splittings in the (1)H dimension of the spe...

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Publicat a:J Biomol NMR
Autors principals: Roche, Julien, Ying, Jinfa, Bax, Ad
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4744140/
https://ncbi.nlm.nih.gov/pubmed/26660434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-015-0004-y
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