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ARTSY-J: Convenient and precise measurement of (3)J(HNH)(α) couplings in medium-size proteins from TROSY-HSQC spectra

A new and convenient method, named ARTSY-J, is introduced that permits extraction of the (3)J(HNHα) couplings in proteins from the relative intensities in a pair of (15)N-(1)H TROSY-HSQC spectra. The pulse scheme includes (3)J(HNHα) dephasing of the narrower TROSY (1)H(N)-{(15)N} doublet component d...

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Bibliografiske detaljer
Udgivet i:J Magn Reson
Main Authors: Roche, Julien, Ying, Jinfa, Shen, Yang, Torchia, Dennis A., Bax, Ad
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4915346/
https://ncbi.nlm.nih.gov/pubmed/27179455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmr.2016.05.001
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