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A high-throughput assay for the comprehensive profiling of DNA ligase fidelity

DNA ligases have broad application in molecular biology, from traditional cloning methods to modern synthetic biology and molecular diagnostics protocols. Ligation-based detection of polynucleotide sequences can be achieved by the ligation of probe oligonucleotides when annealed to a complementary t...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Lohman, Gregory J. S., Bauer, Robert J., Nichols, Nicole M., Mazzola, Laurie, Bybee, Joanna, Rivizzigno, Danielle, Cantin, Elizabeth, Evans, Thomas C.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2016
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4737175/
https://ncbi.nlm.nih.gov/pubmed/26365241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv898
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