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A high-throughput assay for the comprehensive profiling of DNA ligase fidelity

DNA ligases have broad application in molecular biology, from traditional cloning methods to modern synthetic biology and molecular diagnostics protocols. Ligation-based detection of polynucleotide sequences can be achieved by the ligation of probe oligonucleotides when annealed to a complementary t...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Lohman, Gregory J. S., Bauer, Robert J., Nichols, Nicole M., Mazzola, Laurie, Bybee, Joanna, Rivizzigno, Danielle, Cantin, Elizabeth, Evans, Thomas C.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4737175/
https://ncbi.nlm.nih.gov/pubmed/26365241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv898
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