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Guanosine binding to the Tetrahymena ribozyme: thermodynamic coupling with oligonucleotide binding.

The L-21 Sca I ribozyme derived from the group I intron of Tetrahymena thermophila pre-rRNA catalyzes an endonuclease reaction analogous to the first step of self-splicing. Guanosine (G) is bound by the ribozyme, and its 3'-hydroxyl group acts as the nucleophile. Here, we provide evidence that Km fo...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: McConnell, T S, Cech, T R, Herschlag, D
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1993
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47356/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8378306/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.18.8362
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