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Holliday junctions generate super-bright antibodies and antibody fragments in sortase-catalyzed reactions

Site-specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self-quenching of multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site-specifically. Holliday junctions enable modifi...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Angew Chem Int Ed Engl
Päätekijät: Li, Zeyang, Theile, Christopher S., Chen, Guan-Yu, Bilate, Angelina M., Duarte, Joao N., Avalos, Ana M., Fang, Tao, Barberena, Roberto, Sato, Shuji, Ploegh, Hidde L.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2015
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4711269/
https://ncbi.nlm.nih.gov/pubmed/26252716
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201505277
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