A generic strategy for CRISPR-Cas9-mediated gene tagging

Genome engineering has been greatly enhanced by the availability of Cas9 endonuclease that can be targeted to almost any genomic locus using so called guide RNAs (gRNAs). However, the introduction of foreign DNA sequences to tag an endogenous gene is still cumbersome as it requires the synthesis or...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Lackner, Daniel H., Carré, Alexia, Guzzardo, Paloma M., Banning, Carina, Mangena, Ramu, Henley, Tom, Oberndorfer, Sarah, Gapp, Bianca V., Nijman, Sebastian M.B., Brummelkamp, Thijn R., Bürckstümmer, Tilmann
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4703899/
https://ncbi.nlm.nih.gov/pubmed/26674669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms10237
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