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Kinetics of nucleotide-dependent structural transitions in the kinesin-1 hydrolysis cycle

To dissect the kinetics of structural transitions underlying the stepping cycle of kinesin-1 at physiological ATP, we used interferometric scattering microscopy to track the position of gold nanoparticles attached to individual motor domains in processively stepping dimers. Labeled heads resided sta...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Mickolajczyk, Keith J., Deffenbaugh, Nathan C., Ortega Arroyo, Jaime, Andrecka, Joanna, Kukura, Philipp, Hancock, William O.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4702989/
https://ncbi.nlm.nih.gov/pubmed/26676576
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1517638112
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