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Label-free Imaging of Microtubules with Sub-nm Precision Using Interferometric Scattering Microscopy

Current in vitro optical studies of microtubule dynamics tend to rely on fluorescent labeling of tubulin, with tracking accuracy thereby limited by the quantum yield of fluorophores and by photobleaching. Here, we demonstrate label-free tracking of microtubules with nanometer precision at kilohertz...

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Bibliografiske detaljer
Udgivet i:Biophys J
Main Authors: Andrecka, Joanna, Ortega Arroyo, Jaime, Lewis, Katie, Cross, Robert A., Kukura, Philipp
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4806212/
https://ncbi.nlm.nih.gov/pubmed/26745424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.10.055
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