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Label-free Imaging of Microtubules with Sub-nm Precision Using Interferometric Scattering Microscopy

Current in vitro optical studies of microtubule dynamics tend to rely on fluorescent labeling of tubulin, with tracking accuracy thereby limited by the quantum yield of fluorophores and by photobleaching. Here, we demonstrate label-free tracking of microtubules with nanometer precision at kilohertz...

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Detalles Bibliográficos
Publicado en:Biophys J
Autores principales: Andrecka, Joanna, Ortega Arroyo, Jaime, Lewis, Katie, Cross, Robert A., Kukura, Philipp
Formato: Artigo
Lenguaje:Inglês
Publicado: The Biophysical Society 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4806212/
https://ncbi.nlm.nih.gov/pubmed/26745424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.10.055
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