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High-resolution single-molecule kinesin assays at kHz frame rates
This chapter describes methods for high-speed, unloaded, in vitro single-molecule kinesin tracking experiments. Instructions are presented for constructing a total internal reflection dark field microscope (TIRDFM) and labeling motors with gold nanoparticles. An AMP-PNP unlocking assay is introduced...
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| Publicado en: | Methods Mol Biol |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7851763/ https://ncbi.nlm.nih.gov/pubmed/29971716 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8556-2_7 |
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