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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Macromolecular delivery strategies typically utilize the endocytic pathway as a route of cellular entry. However, endosomal entrapment severely limits the efficiency with which macromolecules penetrate the cytosolic space of cells. Recently, we have circumvented this problem by identifying the reage...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Najjar, Kristina, Erazo-Oliveras, Alfredo, Pellois, Jean-Philippe
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692585/
https://ncbi.nlm.nih.gov/pubmed/26382730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53175
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