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Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates
AIM: To develop a culture mode providing durable biomaterials with high yields and activities used in bioartificial liver. METHODS: Hepatocytes were isolated from a whole pig liver by Seglen′s method of orthotopic perfusion with collagenase. In culture on microcarriers, primary porcine hepatocytes w...
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| Pubblicato in: | World J Gastroenterol |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Baishideng Publishing Group Inc
2000
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4688753/ https://ncbi.nlm.nih.gov/pubmed/11819599 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v6.i3.365 |
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