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Simplified isolation and spheroidal aggregate culture of rat hepatocytes
AIM: To explore a simplified method for isolation of hepatocytes and establish a method of primary hepatocyte culture with more aggregates and longer persisence. METHODS: Wistar rat hepatocytes were isolated by a single extracorporeal two-step perfusion method, and the cells were seeded on poly-HEMA...
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| Publicado no: | World J Gastroenterol |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Baishideng Publishing Group Inc
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4767772/ https://ncbi.nlm.nih.gov/pubmed/11819238 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v4.i1.74 |
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