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Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1–RanGTP–SPN1–Nup214 quaternary nuclear export complex

High conformational flexibility is an intrinsic and indispensable property of nuclear transport receptors, which makes crystallization and structure determination of macromolecular complexes containing exportins or importins particularly challenging. Here, the crystallization and structure determina...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Acta Crystallogr F Struct Biol Commun
Päätekijät: Monecke, Thomas, Dickmanns, Achim, Weiss, Manfred S., Port, Sarah A., Kehlenbach, Ralph H., Ficner, Ralf
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: International Union of Crystallography 2015
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666476/
https://ncbi.nlm.nih.gov/pubmed/26625290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15021524
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