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Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1–RanGTP–SPN1–Nup214 quaternary nuclear export complex

High conformational flexibility is an intrinsic and indispensable property of nuclear transport receptors, which makes crystallization and structure determination of macromolecular complexes containing exportins or importins particularly challenging. Here, the crystallization and structure determina...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Monecke, Thomas, Dickmanns, Achim, Weiss, Manfred S., Port, Sarah A., Kehlenbach, Ralph H., Ficner, Ralf
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666476/
https://ncbi.nlm.nih.gov/pubmed/26625290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15021524
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