Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1–RanGTP–SPN1–Nup214 quaternary nuclear export complex

High conformational flexibility is an intrinsic and indispensable property of nuclear transport receptors, which makes crystallization and structure determination of macromolecular complexes containing exportins or importins particularly challenging. Here, the crystallization and structure determina...

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Bibliografiske detaljer
Udgivet i:Acta Crystallogr F Struct Biol Commun
Main Authors: Monecke, Thomas, Dickmanns, Achim, Weiss, Manfred S., Port, Sarah A., Kehlenbach, Ralph H., Ficner, Ralf
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2015
Fag:
Research Communications
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666476/
https://ncbi.nlm.nih.gov/pubmed/26625290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15021524
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