Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1–RanGTP–SPN1–Nup214 quaternary nuclear export complex

High conformational flexibility is an intrinsic and indispensable property of nuclear transport receptors, which makes crystallization and structure determination of macromolecular complexes containing exportins or importins particularly challenging. Here, the crystallization and structure determina...

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Publicado en:Acta Crystallogr F Struct Biol Commun
Autores principales: Monecke, Thomas, Dickmanns, Achim, Weiss, Manfred S., Port, Sarah A., Kehlenbach, Ralph H., Ficner, Ralf
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2015
Materias:
Research Communications
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666476/
https://ncbi.nlm.nih.gov/pubmed/26625290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15021524
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