Base-resolution detection of N(4)-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing

Restriction-modification (R-M) systems pose a major barrier to DNA transformation and genetic engineering of bacterial species. Systematic identification of DNA methylation in R-M systems, including N(6)-methyladenine (6mA), 5-methylcytosine (5mC) and N(4)-methylcytosine (4mC), will enable strategie...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Yu, Miao, Ji, Lexiang, Neumann, Drexel A., Chung, Dae-hwan, Groom, Joseph, Westpheling, Janet, He, Chuan, Schmitz, Robert J.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666385/
https://ncbi.nlm.nih.gov/pubmed/26184871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv738
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