Kinetics of p56lck and p60src Src homology 2 domain binding to tyrosine-phosphorylated peptides determined by a competition assay or surface plasmon resonance.

Src homology 2 (SH2) domains are phosphotyrosine-binding modules found within various signal-transducing proteins. We have determined by 125I competition assay and surface plasmon resonance that the SH2 domains of Src and Lck bind to a variety of phosphopeptides with similar affinity and specificity...

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Опубликовано в::	Proc Natl Acad Sci U S A
Главные авторы:	Payne, G, Shoelson, S E, Gish, G D, Pawson, T, Walsh, C T
Формат:	Artigo
Язык:	Inglês
Опубликовано:	National Academy of Sciences 1993
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46621/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7685110/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.11.4902
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