Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition

Tools for rapid and efficient transgenesis in “safe harbor” loci in an isogenic context remain important to exploit the possibilities of human pluripotent stem cells (hPSCs). We created hPSC master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus that all...

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Bibliografiske detaljer
Udgivet i:Stem Cell Reports
Main Authors: Ordovás, Laura, Boon, Ruben, Pistoni, Mariaelena, Chen, Yemiao, Wolfs, Esther, Guo, Wenting, Sambathkumar, Rangarajan, Bobis-Wozowicz, Sylwia, Helsen, Nicky, Vanhove, Jolien, Berckmans, Pieter, Cai, Qing, Vanuytsel, Kim, Eggermont, Kristel, Vanslembrouck, Veerle, Schmidt, Béla Z., Raitano, Susanna, Van Den Bosch, Ludo, Nahmias, Yaakov, Cathomen, Toni, Struys, Tom, Verfaillie, Catherine M.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2015
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4649136/
https://ncbi.nlm.nih.gov/pubmed/26455413
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2015.09.004
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