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Coupling Binding to Catalysis – Using Yeast Cell Surface Display to Select Enzymatic Activities
We find yeast cell surface display can be used to engineer enzymes by selecting the enzyme library for high affinity binding to reaction intermediates. Here we cover key steps of enzyme engineering on the yeast cell surface including library design, construction, and selection based on magnetic and...
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| Опубликовано в: : | Methods Mol Biol |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2015
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4648535/ https://ncbi.nlm.nih.gov/pubmed/26060080 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2748-7_14 |
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