Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-dependent integration of donor DNA by using TALEN or CRISPR...

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Bibliographic Details
Published in:Int J Mol Sci
Main Authors: Sakuma, Tetsushi, Takenaga, Mitsumasa, Kawabe, Yoshinori, Nakamura, Takahiro, Kamihira, Masamichi, Yamamoto, Takashi
Format: Artigo
Language:Inglês
Published: MDPI 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4632728/
https://ncbi.nlm.nih.gov/pubmed/26473830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms161023849
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