Chemical derivatization of histones for facilitated analysis by mass spectrometry

Histone post-translational modifications have been recently intensely studied owing to their role in regulating gene expression. Here, we describe protocols for the characterization of histone modifications in both qualitative and semiquantitative manners using chemical derivatization and tandem mas...

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Publicado en:Nat Protoc
Autores principales: Garcia, Benjamin A, Mollah, Sahana, Ueberheide, Beatrix M, Busby, Scott A, Muratore, Tara L, Shabanowitz, Jeffrey, Hunt, Donald F
Formato: Artigo
Lenguaje:Inglês
Publicado: 2007
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4627699/
https://ncbi.nlm.nih.gov/pubmed/17446892
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.106
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