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Degradation of C-terminal tag sequences on domain antibodies purified from E. coli supernatant

Expression of recombinant proteins often takes advantage of peptide tags expressed in fusion to allow easy detection and purification of the expressed proteins. However, as the fusion peptides most often are flexible appendages at the N- or C-terminal, proteolytic cleavage may result in removal of t...

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Bibliografische gegevens
Gepubliceerd in:MAbs
Hoofdauteurs: Lykkemark, Simon, Mandrup, Ole Aalund, Friis, Niels Anton, Kristensen, Peter
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Taylor & Francis 2014
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4622476/
https://ncbi.nlm.nih.gov/pubmed/25426869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.36211
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