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Degradation of C-terminal tag sequences on domain antibodies purified from E. coli supernatant

Expression of recombinant proteins often takes advantage of peptide tags expressed in fusion to allow easy detection and purification of the expressed proteins. However, as the fusion peptides most often are flexible appendages at the N- or C-terminal, proteolytic cleavage may result in removal of t...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:MAbs
Asıl Yazarlar: Lykkemark, Simon, Mandrup, Ole Aalund, Friis, Niels Anton, Kristensen, Peter
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Taylor & Francis 2014
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4622476/
https://ncbi.nlm.nih.gov/pubmed/25426869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.36211
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