Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes

AIM: To establish a simplified method for generating peptide-major histocompatibility complex (MHC) class I tetramers. METHODS: cDNAs encoding the extracellular domain of human lymphocyte antigen (HLA)-A*0201 heavy chain (A2) and β(2)-microglobulin (β(2)m) from total RNA extracted from leukocytes of...

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Bibliographic Details
Published in:World J Gastroenterol
Main Authors: He, Xian-Hui, Xu, Li-Hui, Liu, Yi
Format: Artigo
Language:Inglês
Published: Baishideng Publishing Group Inc 2005
Subjects:
Basic Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4615439/
https://ncbi.nlm.nih.gov/pubmed/16015686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i27.4180
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