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Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes
AIM: To establish a simplified method for generating peptide-major histocompatibility complex (MHC) class I tetramers. METHODS: cDNAs encoding the extracellular domain of human lymphocyte antigen (HLA)-A*0201 heavy chain (A2) and β(2)-microglobulin (β(2)m) from total RNA extracted from leukocytes of...
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| Publicat a: | World J Gastroenterol |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Baishideng Publishing Group Inc
2005
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4615439/ https://ncbi.nlm.nih.gov/pubmed/16015686 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i27.4180 |
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