A Novel Dual Expression Platform for High Throughput Functional Screening of Phage Libraries in Product like Format

High throughput screenings of single chain Fv (scFv) antibody phage display libraries are currently done as soluble scFvs produced in E.coli. Due to endotoxin contaminations from bacterial cells these preparations cannot be reliably used in mammalian cell based assays. The monovalent nature and lack...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Xiao, Xiaodong, Chen, Yan, Mugabe, Sheila, Gao, Changshou, Tkaczyk, Christine, Mazor, Yariv, Pavlik, Peter, Wu, Herren, Dall’Acqua, William, Chowdhury, Partha Sarathi
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4607404/
https://ncbi.nlm.nih.gov/pubmed/26468955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0140691
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