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Integration of kinetic isotope effect analyses to elucidate ribonuclease mechanism

The well-studied mechanism of ribonuclease A is believed to involve concerted general acid–base catalysis by two histidine residues, His12 and His119. The basic features of this mechanism are often cited to explain rate enhancement by both protein and RNA enzymes that catalyze RNA 2’-O-transphosphor...

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Detalhes bibliográficos
Publicado no:Biochim Biophys Acta
Main Authors: Harris, Michael E, Piccirilli, Joseph A, York, Darrin M
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4604014/
https://ncbi.nlm.nih.gov/pubmed/25936517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2015.04.022
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