Orientation of the LexA DNA-binding motif on operator DNA as inferred from cysteine-mediated phenyl azide crosslinking.

To address the question how the recognition helix of the LexA repressor is positioned within the major groove of operator DNA we have applied a site-specific photocrosslinking approach using a LexA mutant repressor (LexA-C52) that harbors a single cysteine side chain in position 52, close to the COO...

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Hlavní autoři: Dumoulin, P, Oertel-Buchheit, P, Granger-Schnarr, M, Schnarr, M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1993
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC46014/
https://ncbi.nlm.nih.gov/pubmed/8446625
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