Orientation of the LexA DNA-binding motif on operator DNA as inferred from cysteine-mediated phenyl azide crosslinking.

To address the question how the recognition helix of the LexA repressor is positioned within the major groove of operator DNA we have applied a site-specific photocrosslinking approach using a LexA mutant repressor (LexA-C52) that harbors a single cysteine side chain in position 52, close to the COO...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Dumoulin, P, Oertel-Buchheit, P, Granger-Schnarr, M, Schnarr, M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46014/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8446625/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.5.2030
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