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Orientation of the LexA DNA-binding motif on operator DNA as inferred from cysteine-mediated phenyl azide crosslinking.

To address the question how the recognition helix of the LexA repressor is positioned within the major groove of operator DNA we have applied a site-specific photocrosslinking approach using a LexA mutant repressor (LexA-C52) that harbors a single cysteine side chain in position 52, close to the COO...

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Detalhes bibliográficos
Main Authors: Dumoulin, P, Oertel-Buchheit, P, Granger-Schnarr, M, Schnarr, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC46014/
https://ncbi.nlm.nih.gov/pubmed/8446625
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