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Evidence for an Induced Conformational Change in the Catalytic Mechanism of Homoisocitrate Dehydrogenase for Saccharomyces cerevisiae: Characterization of the D271N Mutant Enzyme

Homoisocitrate dehydrogenase (HIcDH) catalyzes the NAD(+)-dependent oxidative decarboxylation of HIc to α-ketoadipate, the fourth step in the α-aminoadipate pathway responsible for the de novo synthesis of L-lysine in fungi. A mechanism has been proposed for the enzyme that makes use of a Lys-Tyr pa...

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Detalhes bibliográficos
Publicado no:Arch Biochem Biophys
Main Authors: Hsu, Chaonan, West, Ann H., Cook, Paul F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4587348/
https://ncbi.nlm.nih.gov/pubmed/26325079
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2015.08.016
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