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Site-directed Mutagenesis as a Probe of the Acid-base Catalytic Mechanism of Homoisocitrate Dehydrogenase from Saccharomyces cerevisiae
Homoisocitrate dehydrogenase (HIcDH) catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate using NAD as the oxidant. A recent consideration of the structures of enzymes in the same family as HIcDH, including isopropylmalate and isocitrate dehydrogenase...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2760458/ https://ncbi.nlm.nih.gov/pubmed/19530703 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900175z |
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