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Site-directed Mutagenesis as a Probe of the Acid-base Catalytic Mechanism of Homoisocitrate Dehydrogenase from Saccharomyces cerevisiae

Homoisocitrate dehydrogenase (HIcDH) catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate using NAD as the oxidant. A recent consideration of the structures of enzymes in the same family as HIcDH, including isopropylmalate and isocitrate dehydrogenase...

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Autori principali: Lin, Ying, West, Ann H., Cook, Paul F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2760458/
https://ncbi.nlm.nih.gov/pubmed/19530703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900175z
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