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Evidence in Support of Lysine 77 and Histidine 96 as Acid-base Catalytic Residues in Saccharopine Dehydrogenase from Saccharomyces cerevisiae
Saccharopine dehydrogenase (SDH) catalyzes the final reaction in the α–aminoadipate pathway, the conversion of l-saccharopine to l-lysine (Lys) and α-ketoglutarate (α-Kg) using NAD(+) as an oxidant. The enzyme utilizes a general acid-base mechanism to carry out its reaction with a base proposed to a...
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| Glavni autori: | , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2012
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3297426/ https://ncbi.nlm.nih.gov/pubmed/22243403 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201808u |
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