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Evidence in Support of Lysine 77 and Histidine 96 as Acid-base Catalytic Residues in Saccharopine Dehydrogenase from Saccharomyces cerevisiae

Saccharopine dehydrogenase (SDH) catalyzes the final reaction in the α–aminoadipate pathway, the conversion of l-saccharopine to l-lysine (Lys) and α-ketoglutarate (α-Kg) using NAD(+) as an oxidant. The enzyme utilizes a general acid-base mechanism to carry out its reaction with a base proposed to a...

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Bibliografiset tiedot
Päätekijät: Kumar, Vidya Prasanna, Thomas, Leonard M., Bobyk, Kostyantyn D., Andi, Babak, Cook, Paul F., West, Ann H.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2012
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3297426/
https://ncbi.nlm.nih.gov/pubmed/22243403
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201808u
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