Fluorescent ratiometric pH indicator SypHer2: applications in neuroscience and regenerative biology

BACKGROUND: SypHer is a genetically encoded fluorescent pH-indicator with a ratiometric readout, suitable for measuring fast intracellular pH shifts. However, a relatively low brightness of the indicator limits its use. METHODS: Here we designed a new version of pH-sensor - SypHer-2, that has up to...

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Библиографические подробности
Опубликовано в: :Biochim Biophys Acta
Главные авторы: Matlashov, Mikhail E., Bogdanova, Yulia A., Ermakova, Galina V., Mishina, Natalia M., Ermakova, Yulia G., Nikitin, Evgeny S., Balaban, Pavel M., Okabe, Shigeo, Lukyanov, Sergey, Enikolopov, Grigori, Zaraisky, Andrey G., Belousov, Vsevolod V.
Формат: Artigo
Язык:Inglês
Опубликовано: 2015
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4587288/
https://ncbi.nlm.nih.gov/pubmed/26259819
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbagen.2015.08.002
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