Fluorescent ratiometric pH indicator SypHer2: applications in neuroscience and regenerative biology

BACKGROUND: SypHer is a genetically encoded fluorescent pH-indicator with a ratiometric readout, suitable for measuring fast intracellular pH shifts. However, a relatively low brightness of the indicator limits its use. METHODS: Here we designed a new version of pH-sensor - SypHer-2, that has up to...

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Udgivet i:Biochim Biophys Acta
Main Authors: Matlashov, Mikhail E., Bogdanova, Yulia A., Ermakova, Galina V., Mishina, Natalia M., Ermakova, Yulia G., Nikitin, Evgeny S., Balaban, Pavel M., Okabe, Shigeo, Lukyanov, Sergey, Enikolopov, Grigori, Zaraisky, Andrey G., Belousov, Vsevolod V.
Format: Artigo
Sprog:Inglês
Udgivet: 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4587288/
https://ncbi.nlm.nih.gov/pubmed/26259819
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbagen.2015.08.002
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