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Biochemical isolation of Argonaute protein complexes by Ago-APP
During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , , , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4586862/ https://ncbi.nlm.nih.gov/pubmed/26351695 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1506116112 |
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