Biochemical isolation of Argonaute protein complexes by Ago-APP

During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Hauptmann, Judith, Schraivogel, Daniel, Bruckmann, Astrid, Manickavel, Sudhir, Jakob, Leonhard, Eichner, Norbert, Pfaff, Janina, Urban, Marc, Sprunck, Stefanie, Hafner, Markus, Tuschl, Thomas, Deutzmann, Rainer, Meister, Gunter
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2015
Témata:
Biological Sciences
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4586862/
https://ncbi.nlm.nih.gov/pubmed/26351695
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1506116112
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