Biochemical isolation of Argonaute protein complexes by Ago-APP

During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Hauptmann, Judith, Schraivogel, Daniel, Bruckmann, Astrid, Manickavel, Sudhir, Jakob, Leonhard, Eichner, Norbert, Pfaff, Janina, Urban, Marc, Sprunck, Stefanie, Hafner, Markus, Tuschl, Thomas, Deutzmann, Rainer, Meister, Gunter
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 2015
Pynciau:
Biological Sciences
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4586862/
https://ncbi.nlm.nih.gov/pubmed/26351695
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1506116112
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg