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Single-molecule spectroscopy reveals how calmodulin activates NO synthase by controlling its conformational fluctuation dynamics

Mechanisms that regulate the nitric oxide synthase enzymes (NOS) are of interest in biology and medicine. Although NOS catalysis relies on domain motions, and is activated by calmodulin binding, the relationships are unclear. We used single-molecule fluorescence resonance energy transfer (FRET) spec...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: He, Yufan, Haque, Mohammad Mahfuzul, Stuehr, Dennis J., Lu, H. Peter
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4586839/
https://ncbi.nlm.nih.gov/pubmed/26311846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1508829112
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