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Single-molecule spectroscopy reveals how calmodulin activates NO synthase by controlling its conformational fluctuation dynamics

Mechanisms that regulate the nitric oxide synthase enzymes (NOS) are of interest in biology and medicine. Although NOS catalysis relies on domain motions, and is activated by calmodulin binding, the relationships are unclear. We used single-molecule fluorescence resonance energy transfer (FRET) spec...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: He, Yufan, Haque, Mohammad Mahfuzul, Stuehr, Dennis J., Lu, H. Peter
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4586839/
https://ncbi.nlm.nih.gov/pubmed/26311846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1508829112
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