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Using bioluminescent resonance energy transfer (BRET) to characterize agonist-induced arrestin recruitment to modified and unmodified G protein-coupled receptors (GPCRs)

G protein-coupled receptors (GPCRs) represent (~)25% of current drug targets. Ligand binding to these receptors activates G proteins and arrestins, which are involved in differential signaling pathways. Functionally selective or biased ligands activate one of these two pathways and may be superior m...

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Detalhes bibliográficos
Publicado no:Curr Protoc Pharmacol
Main Authors: Donthamsetti, Prashant, Quejada, Jose Rafael, Javitch, Jonathan A., Gurevich, Vsevolod V., Lambert, Nevin A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4583203/
https://ncbi.nlm.nih.gov/pubmed/26331887
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471141755.ph0214s70
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