Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease

Mutation of the tobacco etch virus (TEV) protease nucleophile from cysteine to serine causes an approximately ∼10(4)-fold loss in activity. Ten rounds of directed evolution of the mutant, TEV(Ser), overcame the detrimental effects of nucleophile exchange to recover near-wild-type activity in the mut...

Full description

Saved in:
Bibliographic Details
Published in:Chembiochem
Main Authors: Shafee, Thomas, Gatti-Lafranconi, Pietro, Minter, Ralph, Hollfelder, Florian
Format: Artigo
Language:Inglês
Published: WILEY-VCH Verlag 2015
Subjects:
Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4576821/
https://ncbi.nlm.nih.gov/pubmed/26097079
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201500295
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items