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In vitro affinity screening of protein and peptide binders by megavalent bead surface display

The advent of protein display systems has provided access to tailor-made protein binders by directed evolution. We introduce a new in vitro display system, bead surface display (BeSD), in which a gene is mounted on a bead via strong non-covalent (streptavidin/biotin) interactions and the correspondi...

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Detalhes bibliográficos
Main Authors: Diamante, Letizia, Gatti-Lafranconi, Pietro, Schaerli, Yolanda, Hollfelder, Florian
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3785251/
https://ncbi.nlm.nih.gov/pubmed/23980186
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzt039
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