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Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process
In vitro display technologies have proved to be powerful tools for obtaining high-affinity protein binders. We recently described SNAP display, an entirely in vitro DNA display system that uses the SNAP-tag to link protein with its encoding DNA in water-in-oil emulsions. Here, we apply SNAP display...
Kaydedildi:
| Yayımlandı: | Protein Eng Des Sel |
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| Asıl Yazarlar: | , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
2015
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4550541/ https://ncbi.nlm.nih.gov/pubmed/26134501 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzv029 |
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