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Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process

In vitro display technologies have proved to be powerful tools for obtaining high-affinity protein binders. We recently described SNAP display, an entirely in vitro DNA display system that uses the SNAP-tag to link protein with its encoding DNA in water-in-oil emulsions. Here, we apply SNAP display...

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Bibliografiska uppgifter
I publikationen:Protein Eng Des Sel
Huvudupphovsmän: Houlihan, Gillian, Gatti-Lafranconi, Pietro, Lowe, David, Hollfelder, Florian
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2015
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4550541/
https://ncbi.nlm.nih.gov/pubmed/26134501
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzv029
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