Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.

Wild-type ribonuclease A and five mutants thereof have been expressed in Escherichia coli as fusion proteins by using a T7 expression system. The five mutants are C[65-72]S, C[40-95]S, C[58-110]S, C[26-84]S, and K41G. The expressed fusion protein formed inclusion bodies which were then cleaved by fa...

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Main Authors: Laity, J H, Shimotakahara, S, Scheraga, H A
Format: Artigo
Jezik:Inglês
Izdano: 1993
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC45714/
https://ncbi.nlm.nih.gov/pubmed/8421696
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