Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.
Wild-type ribonuclease A and five mutants thereof have been expressed in Escherichia coli as fusion proteins by using a T7 expression system. The five mutants are C[65-72]S, C[40-95]S, C[58-110]S, C[26-84]S, and K41G. The expressed fusion protein formed inclusion bodies which were then cleaved by fa...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45714/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8421696/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.2.615 |
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